The second group (group II) is composed of samples that express bona fide immediate early genes, e.g., UL123, US3, and UL36, as well as US33A, which is the most highly expressed transcript early in infection (18), and importantly has very limited expression of transcripts that are abundant at the late stage of lytic infection (Fig. 1C and (S2B). This evidence concerns the gene RPS3 and infection.