Congruent with previous OVs using dual gene virotherapy, the study exhibited promising results in colon cancer cells by using manganese superoxide dismutase as a potential tumor suppressor gene along with the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) gene embedded in an Ad platform with a deleted E1B-55 kD region. Here, SOD2 is linked to malignant colon neoplasm.