TGFB1 and neoplasm: Although not determined here, Tregs are known to secrete immuno-inhibitory cytokines, such as IL-10, IL-35 and TGFβ, and interact with DCs in the TME that inhibit T-cell effector functions, induce T-cell anergy, prevent T- and B-cell proliferation, and inhibit NK, DC and macrophage anti-tumor functions [29].