In contrast, CHD3 depletion did not lead to DUX4 de-repression in either control or FSHD cells (Figure 2—figure supplement 4), consistent with its absence from the gD4Z4 enChIP purifications and the mutually exclusive nature of CHD3 and CHD4 within the NuRD complex. This evidence concerns the gene CHD4 and facioscapulohumeral muscular dystrophy.