Combining with CFF‐1‐induced inhibition of PI3K/AKT signaling pathway (Fig. 3A and B), it is clearly that CFF‐1‐induced inhibition of mTOR/p70S6K signal pathway and cell autophagy in LNCaP and PC3 cells was via inhibiting the activity of PI3K/AKT signal pathway, suggesting cell autophagy was involved in CFF‐1‐induced cell growth inhibition and apoptosis in prostate cancer cells. Here, AKT1 is linked to Familial prostate cancer.