To determine that Raf/MEK/Erk signal pathway was involved in cell cycle arrest in G1 phase in CFF‐1‐treated PCa cells, LNCaP and PC3 cells were cultured and treated with different concentrations of CFF‐1 as indicated in Figure 5 for 24 h; then, cells were harvested for Western blot assay to check the protein levels of cell cycle‐related Cyclin D1, p21, and p27. This evidence concerns the gene MAP2K7 and posterior cortical atrophy.