In particular, digestive organ cancer cell lines, such as 58As9 (scirrhous cancer), CaCO2 and HT-29 (colon cancer) expressed the full-length PLEKHN1, and we could ignore the non-specific band by comparison of PLEKHN1-knockout-HT-29 (hereafter, PLEK-KO cells) and the parental HT-29 cells (Fig. 1e). Here, PLEK is linked to cancer.