Here, we determine whether miR-129-5p directly represses Wnt5a expression, which inactivates non-canonical Wnt signalling and leads to the subsequent inhibition of GBM cell proliferation, angiogenesis, epithelial/mesenchymal transition (EMT), invasion, migration, neurosphere formation, chemoresistance and in vivo tumour growth. Here, WNT5A is linked to neoplasm.