SLC22A8 and hepatocellular carcinoma: We examined the in vitro gene-silencing effect of these ASOs by cotransfecting cultured mouse hepatocellular carcinoma (Hepa1–6) cells with a Renilla luciferase-fused OAT3 expression vector and a firefly luciferase expression vector because there were no cultured cells stably expressing endogenous OAT3. Among sequences that effectively inhibited OAT3 expression, we selected sequences No. 1 (93% inhibition), 18 (79% inhibition), and 30 (81% inhibition) (Supplementary Fig. S1).