Of these 11 miRNAs, 6 mRNAs were validated with regard to both the direction of expression changes and to the significance of different expressions between MFS patients and HV controls including 5 significantly up-regulated mRNAs (DYSF, GBP2, LIMK2, MMP9, and MX1) and one significantly down-regulated mRNA (POT1) (Fig. 4). This evidence concerns the gene MMP9 and Marfan syndrome.