We further investigated the effect of TUSC7 on biological behavior of ESCC cells (EC9706 and KYSE30), and found overexpression of TUSC7/inhibition of miR-224 repressed proliferation of ESCC cells, promoted cell apoptosis, and inhibited chemotherapy resistance of ESCC cells by regulating DESC1. The gene discussed is TMPRSS11E; the disease is esophageal squamous cell carcinoma.