As shown in Fig. 4a and b, inhibition rate increased with the increase of concentration of cisplatin or 5-Fu, and overexpression of TUSC7 increased the inhibition effect of cisplatin or 5-Fu on ESCC cells, indicating overexpression of TUSC7 inhibited chemotherapy resistance of ESCC cells. This evidence concerns the gene TUSC7 and esophageal squamous cell carcinoma.