GBM #109-ETV2 (ETV2-overexpressing patient GBM cells, 1 × 106, 21% were infected with Lentivirus-ETV2) or GBM #109 (1 × 106, 19% were infected with control Lentivirus) cells were orthotopically implanted into the brain of NOD/Scid mice (n = 5 in each group) as previously described.21 Two weeks later, the mice were killed, and the brains were sectioned and analyzed by HE as well as multicolor IF. Here, ETV2 is linked to glioblastoma.