To determine whether EGFR-mediated signaling participated in the inhibitory effect of miR-338-5p on ABCB1, we examined the levels of the major components of the EGFR pathway, including p-EGFR, ERK1/2, p-ERK1/2, AKT, p-AKT, JNK, p-JNK, P38, and p-P38, in the HCC cells overexpressing miR-338-5p. This evidence concerns the gene EGFR and hepatocellular carcinoma.