CDH1 and cancer: We then optimised culture conditions including sera, media and substrates, and newly established three cancer cell lines (MDGC7, 8 and 9) with the round shape from primary GC and lymph node metastasis of DGC of the DCKO mice (Fig. 2a), which harboured complete recombination of the Cdh1 and Trp53 loci as expected (Supplementary Figure 1B).