Based on somatic mutations detected by sorted cell sequencing, TP53, EGFR, PTEN, RB1, KRAS, CTNNB1, GNAQ, SMAD4, IDH1, CDKN2A, APC, PIK3CA, HRAS, and NRAS were observed significantly in 22 lung adenocarcinomas (Fig. 5). The gene discussed is EGFR; the disease is lung adenocarcinoma.