Based on somatic mutations detected by sorted cell sequencing, TP53, EGFR, PTEN, RB1, KRAS, CTNNB1, GNAQ, SMAD4, IDH1, CDKN2A, APC, PIK3CA, HRAS, and NRAS were observed significantly in 22 lung adenocarcinomas (Fig. 5). The gene discussed is RB1; the disease is lung adenocarcinoma.