To improve homogeneity of tumor cells and to detect tumor variants for a more accurate cancer diagnosis and research, we analyzed cell populations in FFPE lung adenocarcinoma and sorted the stromal cell population (Keratin−/Vimentin+), the tumor cell population (Keratin+/Vimentin−), and the double-positive cell population (Keratin+/Vimentin+) from 22 FFPE lung adenocarcinoma samples via The DEPArray System (Fig. 2 and Supplementary Fig. S1). Here, VIM is linked to cancer.