The allele frequencies of sorted tumor cell variants were increased by 1.3–10.1 times in three tumor suppressor genes such as TP53, PTEN, and RB1 (Fig. 4a) and by 1.3–2.6 times in three oncogenes such as KRAS, CTNNB1, and EGFR (Fig. 4b). This evidence concerns the gene RB1 and neoplasm.