To validate the heatmap results regarding differences in regulation of these gene isoforms in the offspring with ASD, we compared the offspring’s top up-regulated autism susceptibility genes (such as AGAP1, EFR3A and KAT6A, Fig. 2b) and down-regulated autism susceptibility genes (such as NRXN2, SERPINE1, and BBS4, Fig. 2c). The gene discussed is SERPINE1; the disease is autism.