To validate the data obtained using our qPCR approach in clinical samples, we were able to subject 15 of these samples to targeted deep sequencing (TruSeq Amplicon Cancer Panel, Illumina) giving a measured allele frequency (AF) for the PIK3CA H1047R mutation in each tumour and white blood cell reference sample (Fig. 4C and Supplementary Table 2). Here, PIK3CA is linked to neoplasm.