Importantly, the final differentiated cells exhibited high expression levels of Na+ −taurocholate co-transporting polypeptide (NTCP) and farnesoid X receptor or bile acid receptor (FXR) (Fig. 6e), which are known essential mediators for hepatitis B virus entry of hepatocytes and hence, suggesting this hepatic differentiation system may be used for liver disease modeling of viral hepatitis. This evidence concerns the gene SLC10A1 and animal viral hepatitis.