Toll-like receptor 2/6 plays an important role in the recognition of mycobacterial lipopeptides by inducing the production of pro-inflammatory cytokines (23), so we decided to evaluate the production of cytokines on macrophages stimulated with EV-NS, EV-PMA, EV-fMLF, or EV-TB for 24 h. Here, TLR2 is linked to tuberculosis.