Consistent with previous studies, LPS simulation could causes significant shedding of HS and SDC-1 in both in vivo ARDS mice model (Figures 5 A–F) and in vitro HUVECs model (Figures 5 G–L), Nef pretreatment significantly suppresses the shedding of HS (Figures 5A,D,G,J) and SDC-1 (Figures 5B,F,H,L) either in ARDS mice or in HUVECs. The gene discussed is S100B; the disease is acute respiratory distress syndrome.