In this study, tacrolimus 0.03% ointment used alone has also no obvious effect on human epidermal LC number; however, topical tacrolimus reverses high-dose UVB irradiation-induced epidermal LC reduction by inhibiting LC migration via downregulation TNF-α secretion in epidermal keratinocytes. This evidence concerns the gene TNF and laryngotracheoesophageal cleft.