The results showed that there were no significant differences between unpaired primary and metastatic tumors in the HS values of EGFR (primary tumors: median 1.22, IQR 0.68–1.82, n = 204; metastatic tumors: median 1.25, IQR 0.70–2.49, n = 62; P = 0.405; Fig. 4A) and BRAF (primary tumors: median 0.69, IQR 0.25–0.86, n = 10; metastatic tumors: median 0.61, n = 3; P = 0.866; Fig. 4D). This evidence concerns the gene BRAF and metastatic neoplasm.