In the present study, we used global transcriptomics, PCA and shRNA-based function screening to investigate the roles of RAB39A, CPVL, NUP210, LHX2, KCNG3, SLITRK5, FXYD6 and PRAME expression in cancer cells, including CSCs, under both acidic and neutral microenvironmental conditions. Here, FXYD6 is linked to cancer.