Consistent with our previous data (Figure 1C), mice that received saline or a prime immunization with the sH1N1 vaccine did not generate significant neutralizing antibodies (Figure 5A); in addition, we found that the viral loads were 1,000-fold higher in saline and sH1N1 groups compared to responses in the sH1N1 + GLA-SE group at days 3 and 6 following influenza infection (Figure 5B). This evidence concerns the gene GLA and influenza.