Coadministration of tNPs containing rapamycin with free biologic drugs was effective in preventing ADAs against coagulation FVIII (Advate®) in a model of hemophilia A (66, 67); human TNFα-blocking antibody adalimumab (Humira®) in a model of inflammatory arthritis (30), acid-α-glucosidase (Lumizyme®) in a model of Pompe disease (70), recombinant immunotoxin in a model of mesothelioma (71), adeno-associated virus gene therapy vectors (68) and pegylated uricase (pegsiticase) in uricase-deficient mice and non-human primates (30). Here, F8 is linked to hemophilia A.