When evaluating TDP-43 aggregation, we found no significant difference in either the number of neurons with cytoplasmic TDP-43+ puncta or the number of puncta per neuron in any of the ALS iPSC lines compared to control (Figure 3A), which is in contrast to a previous report that showed more inclusions in TDP-43 iPSC-derived cultures (Egawa et al., 2012). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.