The up-regulation of timp2b (4.4-fold) could either activate the pro-inflammatory NF-κB pathway in human melanoma cells, protecting cells from apoptosis40, or exert the anti-inflammatory function by inhibiting NF-κB activity in murine microglial cells, to suppress the production of nitric oxide, TNFα, IL1β, and reactive oxygen species (ROS)41. This evidence concerns the gene TNF and melanoma.