GLS and cancer: These results are consistent with previous findings that glutamine-dependent NSCLC cells are more sensitive to GLS1 inhibition.33 We showed that the expression level of GAC varied between different NSCLC cells, some cancer cells showed higher expression of GAC while some showed even lower expression when compared with bronchial epithelia cells.33,34 However, we demonstrated that the GAC activity in NSCLC cells was much higher than that in HBE cells, indicating the dependence of NSCLC cells on glutamine.