As a serine–threonine kinase, overexpression of PKCε has been demonstrated to be a hallmark of multiple cancers, including breast cancer, prostate cancer, and lung cancer.36–39 In NSCLC cells, PKCε is upregulated and knockdown of PKCε impairs tumor growth.39 However, the molecular mechanism by which PKCε regulates cancer metabolism has not been defined until our current work. This evidence concerns the gene PRKCE and breast cancer.