Knocking down of CD44 provedthe essential role of CD44 in the formation of colonies, liver spheroid, andinvasive ability in vitro, mimicking the results obtained from the inhibition ofTGFβ1 by galunisertib, where the drug reducedthe expression of CD44 and hence decreased the functionality of HCC cells. The gene discussed is CD44; the disease is hepatocellular carcinoma.