Using the same approach as the B6→129 model but including additional TE from the bone marrow (BM), we obtained a total of 42 samples from GVHD mice and syngeneic F→F BMT controls (3 replicate samples/tissue from 3 independent experiments, pooling where necessary from multiple mice from individual experiments), with a median CD8+ T cell purity of 98.6% (range 95.2%–98.8%) and median cell number/sample of 4.1 × 104 (range 0.4 × 104 to 25 × 104). The gene discussed is CD8A; the disease is graft versus host disease.