Third, at the transcriptomic level, we highlighted the up-regulation of several genes encoding major enzymes catalyzing steps in the FA synthesis process (i.e., ACACA, FASN, SCD, ELOVL6, ACLY, ME1); FA activation and transport (ACSL3, ACSL5, ACSL6, MFDS2A, FABP7); and TG synthesis, remodeling and packing to VLDL for export to peripheral organs (GPAT1, AGPAT2, LPIN1, DGAT2, PNPLA3, LIPG, PLIN2, MTTP) in the liver of chickens fed the HF diet (Fig. 7). The gene discussed is AGPAT2; the disease is hydrops fetalis.