We knocked down PKM2 (Figure 3A‐B) and found that down‐regulation of PKM2 enhanced the inhibitor effect of THP on bladder cancer cells detected by MTT (Figure 3C), colony formation assay (Figure 3D) and fluorescence microscopy approach (Figure 3E), indicating the inverse role of PKM2 on THP sensitivity to bladder cancer cells. This evidence concerns the gene PKM and urinary bladder carcinoma.