As depicted in the tables 1 and 4, a key gene profile including eight central genes (GAPDH, PRDM10, TP53, AKT1, INS, ALB, SRC and MAPK1) is highlighted in the PPI network of the rat model of NAFLD after two weeks feeding with high fat diet. This evidence concerns the gene MAPK1 and metabolic dysfunction-associated steatotic liver disease.