We examined ITGA3, ITGB4, ITGB5, and LAMC2 expression in ESCC cell lines and found that LAMC2 and ITGB4 were overexpressed in ESCC cell lines, especially LAMC2, while ITGA3 and ITGB5 were not (Supplementary Figure 1E, F). This evidence concerns the gene ITGB5 and esophageal squamous cell carcinoma.