Next, the RT-qPCR results indicated that LAMC2 was overexpressed in 89.4% (59/66) of ESCC tissues (Fig. 4c), and high LAMC2 expression was associated with primary tumor invasion depth, lymph node metastasis, and advanced TNM stage (Fig. 4d, e, f). The gene discussed is LAMC2; the disease is esophageal squamous cell carcinoma.