Previously studies showed that the lack of expression of gB, gH, or gL alone has drastically effect on viral maturation, which is necessary for viral replication, while HSV-1, EHV-1 or other alpha-herpesvirus could replicate and produce infection progeny virus without the expression of other glycoproteins, such as such as gE, gI, gK and so on22–24. This evidence concerns the gene GNAI1 and infection.