There was no significant change in the expression of SMAD2 and EMT markers and cellular morphology after TGF-β stimulation, despite the overexpression of miR-190 (Fig. 6a and b), suggesting that SMAD2 inhibition mediates the effects of miR-190 overexpression on breast cancer cell EMT. The gene discussed is TGFB1; the disease is breast carcinoma.