Given that RhoH is expressed in PC3 cells, we investigated whether other cancer cell lines used in our laboratory also expressed RhoH. RNA analysis by polymerase chain reaction (PCR) detected RhoH expression in PC3 cells and MCF7 breast cancer cells but not DU145 prostate cancer cells or MDA-MB-231 breast cancer cells (Fig. 4a). Here, RHOH is linked to breast cancer.