Next, we performed immunostaining for epithelial marker E-Cadherin and mesenchymal marker Vimentin and found that CD133+ ICC cells underwent EMT-like alteration characterized by lower E-Cadherin and higher Vimentin expression, indicating that EMT might be a mechanism linking CD133 expression with poor prognosis. This evidence concerns the gene CDH1 and intrahepatic cholangiocarcinoma.