In this study, we generated HGF-gene modified hBMSCs using rAd-HGF and tested the effects of low MOI (MOI = 10 or 50) and high MOIs (MOI = 250 or 1250) on the expression of HGF which was thought to be critical regulator in ANFH therapy. This evidence concerns the gene HGF and familial avascular necrosis of femoral head.