In females, the three clusters of non-frail, pre-frail and frail subjects also shared many similarities, however non-frail subjects had younger age, a greater percentage of normal BMI, more hormone replacement therapy, less family history of heart attack, less hospitalization history of over three times, less vitamin D insufficiency, less high C-reactive protein (CRP), more physical inactivity over 600 MET and shorter sleep duration compared with pre-frail and frail subjects (Tables 1 and 2). This evidence concerns the gene CRP and vitamin D deficiency.