Overexpression of Emx2 in U87MG and T98G cell lines and five GBM cultures from patients, in which Emx2 was selectively activated in only the tumor cells without effect the health cells and was TetON controlled by doxycycline, caused the expansion of the cells arrested for proliferation, significant alteration on the expression of genes related with mitogenic and RTK signaling, and the genes controlling early G1 checkpoint in five GBM patient cultures, and caused the cultures collapsed within 7–8 days after treatment. The gene discussed is EMX2; the disease is neoplasm.