ESCC tissue (GRP94 expression score: mean ± standard deviation, 201.6 ± 48.4) exhibited significantly higher GRP94 expression than normal esophageal squamous epithelial tissue (GRP94 expression score: 98.2 ± 50.0) (P < 0.001). This evidence concerns the gene HSP90B1 and esophageal squamous cell carcinoma.