We observed that treated with AKT inhibitor (perifosine, 10 μM) and COX-2 (celecoxib, 10 μM) to CE81T cells significantly inhibited migration and invasion at 12 and 48 h, indicating that GRP94 regulated AKT activation and COX-2 levels in ESCC cells to facilitate cancer progression and metastasis. The gene discussed is AKT1; the disease is cancer.