We examined those possible KRAS-dependent effects on calpain-2 nucleolar localization in two isogenic human CRC cell lines harboring the same PI3K activating mutation but with a different KRAS mutational status by deletion of either, the wild-type (DMUT) or the mutant allele (DWT7) found in KRASG13D/WT DLD-1 cells. This evidence concerns the gene CAPN2 and colorectal carcinoma.