AKT1 and hepatocellular carcinoma: The effects of mTORC1 inhibition on AKT phosphorylation varied between cell lines: RAD001 drastically increased both P-AKTT308 and P-AKTS473 in ZR75-1 and MCF-7 cells, but only robustly increased P-AKTS473 in HCC-1428 cells (Figure 1B and Supplementary Figure 2).