IGFBP3 and hemoglobin measurement: In particular, the silencing of the TSGs hedgehog-interacting protein (HHIP), insulin-like growth factor-binding protein 3 (IGFBP3), and secreted frizzled-related protein 1 (SFRP1) have been shown to deregulate pivotal pathways of embryonic development, thus promoting HB pathogenesis [9, 10].