To confirm that YFP–SNRPN localised correctly at steady state to CBs and speckles, and to determine where SNRPN localised during maturation and incorporation into snRNPs, the plasmid was transiently expressed in SH-SY5Y neuroblastoma cells, with cells fixed and immunostained at 24 h intervals. Here, SNRPN is linked to neuroblastoma.