Moreover, in liver orthotopic xenograft tumor models, IVIS imaging showed that tumors in HCCLM3CASZ1 group had weaker fluorescence signals than those in HCCLM3control group, however, tumors from PLC/PRF/5shCASZ1 group exhibited stronger fluorescence signals than those from PLC/PRF/5shCtr group (Fig. 4c), which indicated that CASZ1 could inhibit HCC growth and metastasis in vivo. This evidence concerns the gene CASZ1 and neoplasm.