To investigate whether TNFα-mediated Ca2+influx was associated with mitochondrial-dependent intrinsic apoptosis, we evaluated the mitochondrial membrane potential (MMP), the subcellular location of cytochrome c (Cyto c), and the translocation of Bcl-2 family members in HCC cells after TNFα treatment. The gene discussed is BCL2; the disease is hepatocellular carcinoma.