TNF and hepatocellular carcinoma: Moreover, our data indicated that the treatment with a combination of 100 ng/mL TNFα and 1 μM ionomycin exhibited an apoptosis percentage of about 37% in HCC cell HLF, but only about 14% in normal hepatocyte QSG-7701, which is two times as treatment with TNFα (100 ng/mL) only (Additional file 4: Figure S3d, e).