HNF4A and colorectal cancer: In order to assess whether our 90 DEGs had been previously identified as direct targets of β-catenin/TCF-/Lef in other cell types, we compared our gene list with published target genes of canonical Wnt signalling, based on using ChIPseq in U87 glioma cells [37], HEK293, and the colorectal cancer cell line LS174T [38].