To understand the contribution of GSK3 as a key molecule in the anti-hypertrophic signaling regulated by SIRT2, we treated SIRT2 overexpressing cardiomyocytes with GSK3 inhibitors, LiCl (Bertsch et al., 2011) and GSK3 inhibitor X (Li et al., 2014; Meijer et al., 2003) and studied the ISO-induced cardiac hypertrophy. Here, SIRT2 is linked to cardiac hypertrophy.