In conclusion, we demonstrate here that Glo1 sustains metastatic phenotype of DU145 and PC3 cells by controlling EMT in a novel mechanism involving the tumour suppressor miR‐101, MG‐H1‐AP and TGF‐β1/Smad signalling, thus providing valuable new insights into the pathogenesis of advanced PCa and novel options for the development of preventive and therapeutic strategies. Here, DHCR7-DT is linked to neoplasm.